Welcome to the MUTUSLAB
Department of Chemistry & Biochemistry
PI: Dr. Bulent Mutus, PhD (U. Manitoba 1981)
Professor and U.
phone: 519 253 3000 ext. 3533
We study type 2 diabetes-mediated changes in nitric oxide (NO) and redox signaling pathways in blood platelets and endothelial cells, with the objective of lowering the susceptibility of persons with type 2 diabetes to thrombotic diseases.
1. Imaging/Analytical probe development for NOx and thiols.
2. Role of plasma membrane composition in NO biosynthesis and diffusion.
3. Role of plasma forms of protein disulfide isomerase (PDI) in diabetes-related prothrombotic activity.
4. Role of ER stress in plasma membrane lipid composition.
5. Conformational constraints in PDI-substrate recognition.
- Arun Raturi is now a Post-Doctoral Fellow in Dr. Wolfram Ruf’s lab at the Scripps Research Institute.
- Shane Miersch is now a Post-Doctoral Fellow in Dr. Joshua LaBaer’s Lab at the Harvard Institute of Proteomics
Current Research Group:
Ms. Ruchi Chaube firstname.lastname@example.org
Ms. Harman Kaur email@example.com
Ms. Suzie Durocher firstname.lastname@example.org
Ms. Rebecca Heeney email@example.com
Ms. Vasantha Kallaunta firstname.lastname@example.org
Mr. Adam Faccenda email@example.com
Miersch S, and Mutus B. (2007) Membrane Lipid Domains: Techniques for Visualization and Characterization. Current Analytical Chemistry, 3, 81-87.
Raturi, A and Mutus B. (2007 ) Characterization of redox state and reductase activity of protein disulfide isomerase under different redox environments using a sensitive fluorescent assay. Free Radic Biol Med. 43, 62-70.
Miersch, S, Sliskovic I, Raturi A, Mutus B. (2007) Antioxidant and antiplatelet effects of rosuvastatin in a hamster model of prediabetes. Free Radic Biol Med. 42, 270-279.
Kozarova, A, Sliskovic, I, Simon, SS,
Andrews, PC, Mutus, B, Vacratis,
Sliskovic, I, Mutus, B. (2006) Reversible inhibition of caspase-3 activity by iron(III):potential role in physiological control of apoptosis. FEBS Lett. 580, 2233-2237.
Miersch S, Mutus, B. (2005) Protein S-nitrosation: biochemistry and characterization of protein thiol-NO interactions as cellular signals. Clin Biochem. 38, 777-91.
Raturi A, Vacratsis, PO, Seslija, D, Lee, L, Mutus B. (2005) A direct, continuous, sensitive assay for protein disulphide-isomerase based on fluorescence self-quenching. Biochem J. 391, 351-7.
Sliskovic I, Raturi, A and Mutus B. (2005) Characterization of the S-Denitrosation Activity of Protein-Disulfide Isomerase. Journal of Biological Chemistry. 280, 8733-8741.
Al-Kassim, L, Wang,
Z, Szabo, AG,
Root, P, Sliscovic, I, and Mutus, B. (2004) Platelet Cell Surface Protein Disulfide Isomerase-Mediated S-Nitrosothiol Consumption. Biochemical J. 382, 575–580.
Raturi, A and Mutus, B. (2004) Use of 2,3-Diaminonapthalene for studying denitrosation activity of protein disulfide isomerase. Anal. Biochem. 326, 281-283.
Akhter, S, Green, JR, Root, P, Thatcher, GJ and Mutus, B. (2003) Peroxynitrite and NO+ form coloured nitrite adducts with sinapinic acid: potential applications. Nitric Oxide 8, 214-221.
Root, P. and Mutus, B O-Aminobenzoyl-S-nitrosohomocysteine, a fluorogenic probe for cell surface thiol determinations via a microtiter plate assay. (2003) Anal. Biochem. 320, 299-302.
Akhter S, Vignini A, Wen Z, English A, Wang PG, Mutus B. Evidence for S-Nitrosothiol-dependent changes in fibrinogen that do not involve transnitrosation or thiolation. (2002) Proc. Natl. Acad. Sci. USA. 99, 9172-9177.
Chen, X Wen, Z, Xian, M. Wang, K, Ramachandran, N, Tang, X, Schlegel, HB, Mutus, B and WangPG. Fluorophore-Labeled S-Nitrosothiols (2001) J. Org. Chem. 66, 6064-73.
Ramachandran, N, Root, P,Jiang X-M, Hogg, PJ and Mutus B. Mechanism of Transfer of NO from Extracellular S-Nitrosothiols Into the Cytosol by Cell-surface Protein Disulfide Isomerase(2001) Proc. Natl. Acad. Sci. USA. 98,9539-9544.
Mutus, B. Rabini, RA, Staffolani, R, Ricciotti, R, Fumelli, P, Moretti, N, Martarelli D, and Mazzanti L. (2001) Platelets from diabetic subjects show increased sensitivity to homocysteine-induced inhibition of NO production. Diabetologia. 44, 979-982.